Isolation of anti-CD22 Fv with high affinity by Fv display on human cells
نویسندگان
چکیده
منابع مشابه
ISOLATION BY PHAGE DISPLAY OF SINGLE-CHAIN Fv ANTIBODIES AGAINST Escerichia coli K99 FIMBRIAE
Single-chain antibodies (scFv) are small, recombinant proteins in which variable domains from immunoglobulin heavy and light chains are physically linked with a flexible peptide. They are monovalent and lack the heavy chain constant domins for activation of complement and binding to cellular receptors. However scFvs can possess biological activity by binding to and inhibiting the action of thei...
متن کاملCrystal structure of an Fv-Fv idiotope-anti-idiotope complex at 1.9 A resolution.
Anti-idiotopic antibodies react with unique antigenic features, usually associated with the combining sites, of other antibodies. They may thus mimic specific antigens that react with the same antibodies. The structural basis of this mimicry is analyzed here in detail for an anti-idiotopic antibody that mimics the antigen, hen egg-white lysozyme. The crystal structure of an anti-hen-egg-white l...
متن کاملCrystallization and preliminary X-ray diffraction study of an idiotope-anti-idiotope Fv-Fv complex.
A complex between the Fv fragment of an anti-hen eggwhite lysozyme antibody (D1.3) and the Fv fragment of an antibody specific for an idiotypic determinant of D1.3 has been crystallized in a form suitable for X-ray diffraction analysis. Both Fv fragments were expressed in soluble form in Escherichia coli and purified by affinity chromatography; diffraction-quality crystals were only obtained fo...
متن کاملTargeted therapy against human lung cancer in nude mice by high-affinity recombinant antimesothelin single-chain Fv immunotoxin.
Several tumors, including mesothelioma and ovarian cancer, can overexpress mesothelin, a glycosylphosphatidylinositol-linked differentiation glycoprotein. The membrane-bound type of mesothelin is found in the blood of cancer patients at a very low level, which makes mesothelin a good candidate for targeted therapy of certain cancers. An antimesothelin disulfide-linked Fv (SS1 Fv) was fused to a...
متن کاملHigh affinity restricts the localization and tumor penetration of single-chain fv antibody molecules.
Antitumor monoclonal antibodies must bind to tumor antigens with high affinity to achieve durable tumor retention. This has spurred efforts to generate high affinity antibodies for use in cancer therapy. However, it has been hypothesized that very high affinity interactions between antibodies and tumor antigens may impair efficient tumor penetration of the monoclonal antibodies and thus diminis...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2006
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0603653103